Fine mapping of dep1. Two F2 populations were bred from the japonica _ indica crosses (Q169 _ 93-11 and W101 _ NJ 6). The 1,521 segregating individuals with non-erect panicles at maturity were genotyped to identify the homozygous recombination plants for DEP1. Details of the markers used for genotyping are given in Supplementary Table 1. The genomic location of the DEP1 locus was defined by the two molecular markers S2 and S11-2. A search for candidates for dep1 was carried out by comparing the genomic DNA sequences of W101, Q169, 93-11, NJ 6 and Nipponbare.